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1. What kinds of samples are suit for the Elisa kits testing? The common samples are Serum, blood, plasma, Urine, cell culture supernatant and Tissue samples. For more kinds or specific one, please contact our sales representative. 2. How do I prepare the sample for the testing? Different samples have different preparing method. It has detailed introduction of kinds of samples preparing in the product manual. You could consult our sales representatives for the manual or consult them directly. 3. What sizes of the Elisa kits? And how many samples could be tested for each size? GenAisa offers two sizes of elisa kits of 96T and 48T. The 48T elisa kits could test 40 samples and the 96T could test 88 samples at most. 4. Do I have to run all of my standards and samples in duplicate? Yes. To run duplicates in order to compare, to monitor assay precision and to increase the confidence in the assay results obtained. 5. Do I have to run all of my samples one time? No. Each Elisa kit has Micro Elisa Strip plate which could be taken apart to be with different number of wells. This allows the user to analysis different amount of samples at different times. Also users could test two 48T Elisa kits instead of one 96T Elisa kit. 6. Is it possible to store the reagents other than indicated? Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test for improper conditions of storage may cause the inactivation of the antibody. 7. How should I store my samples? Samples should be stored at -20℃ or lower temperature. For long-term storage, it is recommended to freeze them at -70℃-80℃ .Avoid repeated freeze-thaw cycles. 8. What type of reproducible results are obtained with the assays? Each kit comes with a manual containing a graph of typical data obtained. Any variation in operation, pipetting and washing technique, incubation time or temperature, kit age and different samples can cause variation in result. Each user should obtain his own standard curve. However the standard curve should differs not much. 9. Can I use a sample type that is not recommended in the kit insert? The kits have been validated for the sample types listed in the kit inserts as most Elisa kits for human and animals are suit for serum, blood plasma, and other related tissue Liquid. GenAsia Elisa kits are validated for a relatively large range of sample type. Contact Technical Service for further information about the sample types other than those validated for specific product. 10. Can I alter the volume of sample I use in the assay? It is not recommended that you alter the volumes since all GenAsia kits are designed for optimal performance at the given volumes. 11. Can I modify the protocol? NO. The procedures and methods of GenAsia ELISA kits have been optimised to provide the best possible results. Modifying the format or protocol may give inaccurate and wrong results. 12. My samples generated values that were outside the dynamic range of the assay. Can I use these values? It is not recommended to use it. Only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Make sure the samples are within the range of the testing kits. 13. Do I have to run a Blank, or Zero Standard every time? Yes, these are required for the calculations, and reflect any subtle but significant performance changes from day to day and assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem. 14. Can components from different kits be used? Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally as well as with all of the other components in the kit. It is never recommended to use your own components or components from other kits or vendors. 15. My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why? The sample may not contain the analyte or the sample is not validated. A matrix effect may be masking the detection. Ensure the range of the samples are within the range of the testing kits. 16. How do you recommend I wash my plate? If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, and that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. It is recommended to wash the plate 3 to 5 times. The user should be careful to ensure that all of the contents are aspirated and the plate tapped dry on lint-free paper. 17. Do I need to use a plate shaker? Reliable results can be obtained without a plate shaker, but the O.D.'s will generally be lower than those obtained using a plate shaker. 18. Why do I have to use wavelength correction between 450-570nm? For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp and optical fluctuations. 19. What is the expected concentration of analyte that I should expect to find? The amount of a given analyte may vary not only from species-to-species, but also between tissue and cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases. 20. What are the reasons for High Background? Improper Washing: Check volume of washing buffer reservoir and make sure all recommended washing steps are performed.Increase number of washes. Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidising reagents, before use. Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate. Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times and temperatures. However, if all wells are intensely and equally coloured with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the colour is developing, in order to stop the reaction sooner. 21. My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why? The optical density is affected by a number of physical conditions such as time and temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate. 22.why shouldn’t I dilute the sample? GenAsia Elisa kits are optimized of one step method. It is designed to leave out the sample dilution procedure which is easier and quicker to conduct. When diluting, it changes the reacting environment and violate the one step method designed. |
